Biochem/physiol Actions
作用方式：本品通过诱导原核生物中m-RNA模板的误
抗菌谱：潮霉素B对细菌、真菌和高等真核细胞有抑制作
General description
• Formula: C20H37N3O13
• LD50: 6 mg/kg in mouse (i.v.);13 mg/kg in guinea pig (i.p.); 63 mg/kg in rat (i.p.)
• Molecular weight: Mr = 527.5
• Working concentration: 50 - 1,000 μg/ml in cell culture. A commonly used concentration for the selection of mammalian cells is 200 μg/ml. However, the optimal concentration must be experimentally determined, since it may vary depending on the type of cell used.
Other Notes
Solution, 50 mg/ml, in PBS (phosphate-buffered saline), filtered through 0.2 μm pore size membrane.
仅用于生命科学研究。不可用于诊断。
Preparation Note
Working concentration: Recommended concentration for the selection of resistant cells is 50 to 1000 μg/ml in cell culture. A commonly used concentration for the selection of mammalian cells is 200 μg/ml. However, the optimal concentration must be determined experimentally, and may vary with the type of cell used.
Quality
Purity: >80% (HPLC)
Unit Definition
Unit Assay: Unit/mg
We do not have this information.
Physical form
Solution, 50 mg/ml in PBS (phosphate-buffered saline), filtered through 0.2 μm pore size membrane
Application
Hygromycin B is an aminoglycosidic antibiotic that inhibits protein synthesis in prokaryotes and eukaryotes. It is used to select and maintain the phenotype of eukaryotic cells that are stably transfected with the E. coli hygromycin-resistance gene (hyg or hph).[1][2][3]
