Product: Cy ™ 3 - conjugated AffiniPure Donkey Anti - Rat IgG (H+L) (minimal cross - reaction to Bovine, Chicken, Goat, Guinea Pig, Syrian Hamster, Horse, Human, Rabbit, and Sheep Serum Proteins) Code Number: 712 - 165 - 150 Lot Number: 146156 Physical State: Freeze - dried solid Size: 0.5 mg (This product has been overfilled to ensure total recovery of stated quantity . ) Antibody Concentration: 1.5 mg/ml Suggested Dilution Range: 1:100 - 1:800 for most applications Fluorophore: Cy3 bis - NHS ester Amax = 550 nm; Emax = 570 nm Fluorophore/Protein Absorbance Ratio: A552/A280 = 2.70 Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG - Free, Protease - Free) Preservative: 0.05% Sodium Azide Storage and Rehydration: Store freeze - dried solid at 2 - 8 ° C. Rehydrate with 0.4 ml dH2O and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2 - 8 ° C as an undiluted liquid. Extended Storage after Rehydration: Aliquot and freeze at - 70 ° C or below. Avoid repeated freezing and thawing . Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at - 20 ° C as a liquid. Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use. Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Antibody Specificity: Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rat IgG. It also reacts with the light chains of other rat immunoglobulins. No antibody was detected against non - immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid - phase adsorbed to ensure minimal cross - reaction with bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, rabbit, and sheep serum proteins, but it may cross - react with immunoglobulins from other species. Country of Origin: USA Note: For in vitro research use only, not for diagnostic or therapeutic use. This product is not a medical device. Cy is a trademark of GE Healthcare Bio - Sciences Ltd. Jackson ImmunoResearch is licensed by GE Healthcare Bio - Sciences Ltd. to manuf acture and sell conjugates of Cy 2, Cy 3, and Cy 5 reactiv e dy es under US Patent Number 5,268,486 and other patents pending |
