Product: Peroxidase - conjugated AffiniPure Goat Anti - Rabbit IgG (H+L) Code Number: 111 - 035 - 003 Lot Number: 146671 Physical State: Freeze - dried solid Size: 2.0 ml Antibody Concentration: 0.8 mg/ml Suggested Dilution Range: 1:500 - 1:5,000 for immunohisto/cytochemistry 1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates 1:10,000 - 1:200,000 for Western blotting with ECL substrates Peroxidase: Purified from horseradish root Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG - Free, Protease - Free) Preservative: None (Warning: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase. ) Storage and Rehydration: Store freeze - dried solid at 2 - 8 ° C. Rehydrate with 2.0 ml dH2O and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2 - 8 ° C as an undiluted liquid. Extended Storage after Rehydration: Aliquot and freeze at - 70 ° C or below. Avoid repeated freezing and thawing . Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at - 20 ° C as a liquid. Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use. Warning: Confirm that glycerol is ACS grade or better before adding to this product. Lower grades of glycerol may seriously inhibit peroxidase enzyme activity . Purity: The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Antibody Specificity: Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule rabbit IgG. It also reacts with the light chains of other rabbit immunoglobulins. No antibody was detected against non - immunoglobulin serum proteins. The antibody may cross - react with immunoglobulins from other species. Country of Origin: USA Note: For in vitro research use only, not for diagnostic or therapeutic use. This product is not a medical device. |
