DescriptionDulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of Gibco® DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.This DMEM is modified as follows:| With | Without | | • Low Glucose | • Sodium Bicarbonate | | • L-glutamine | • HEPES | | • Sodium Pyruvate | |
The complete formulation is available.
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco® DMEM is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable Gibco® DMEM product made in our Scotland facility (31600-083). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals. Specifications| Form: | Powder |
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| Glucose: | Low Glucose |
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| Glutamine: | L-Glutamine |
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| HEPES Buffer: | No HEPES |
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| Phenol Red Indicator: | Phenol Red |
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| Product Line: | Gibco® |
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| Product Size: | 10 x 1 L |
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| Serum Supplementation: | Standard Serum Supplementation |
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| Shipping Condition: | Room Temperature |
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| Sodium Bicarbonate Buffer: | No Sodium Bicarbonate |
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| Sodium Pyruvate Additive: | Sodium Pyruvate |
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Contents & storageStorage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture Media formulations31600 - DMEM, powder, low glucose, pyruvate |
