DescriptionDMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM/F-12 is modified as follows:
| With | Without | | • L-glutamine | • Sodium Bicarbonate | | • HEPES | |
The complete formulation is available.
Using DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH. Powder forms of Gibco® cell culture media require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
cGMP manufacturing and quality system
DMEM/F-12 is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable DMEM/F-12 product made in our Scotland facility (42400-010). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals. Specifications| Form: | Powder |
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| Glucose: | High Glucose |
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| Glutamine: | L-Glutamine |
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| HEPES Buffer: | HEPES |
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| Phenol Red Indicator: | Phenol Red |
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| Product Line: | Gibco® |
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| Product Size: | 10 x 1 L |
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| Serum Supplementation: | Standard Serum Supplementation |
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| Shipping Condition: | Room Temperature |
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| Sodium Pyruvate Additive: | Sodium Pyruvate |
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Contents & storageStorage conditions: 2-8°C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture Media formulations12400 - DMEM/F-12, powder, HEPES |
